Mouse monoclonal [B3] antibody to TCR V gamma 9 (PE).
Specificity
This antibody recognizes an extracellular epitope of human TCR V gamma 9, a variant of TCR gamma chain, which is expressed on a gamma/delta T cell subpopulation with cytolytic activity against various tumor cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Human T cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
B3
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human TCR V gamma 9 positive lymphocytes (red-filled) from human TCR V gamma 9 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained with Anti-TCR V gamma 9 Antibody [B3] (PE) (10 µl reagent / 100 µl of peripheral whole blood).
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