Anti-TDP43 Antibody [3H8] (A85389)

Immunofluorescent analysis of rat hippocampus section stained with Anti-TARBDP Antibody, at a dilution of 1:2,000, in red, and Anti-GFAP Antibody (A85307 | 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies. The TDP43 protein is concentrated in the nuclei of hippocampal neurons, while the Anti-GFAP Antibody stains the network of astroglial cells.

Anti-TARBDP Antibody was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Anti-GFAP Antibody (A85307 | green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The Anti-TARDP Antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TDP43 and blue due to their content of DNA, stained blue with DAPI.

Western blot analysis of whole brain lysates and nuclear extract from whole brain using Anti-TARBDP Antibody [3H8] (A85389), at a dilution of 1:2,000, in green. The lanes contain samples of: [Lane 1] Protein standards, in red, [Lane 2] rat brain, [Lane 3] rat brain nuclear extract, [Lane 4] mouse brain, [Lane 5] mouse brain nuclear extract. The strong band at 43 kDa visible in both whole brain and brain nuclear extract corresponds to TARBDP protein.

Immunohistochemistry analysis of a 4% PFA fixed paraffin embedded rat cerebellum section with Anti-TDP43 Antibody [3H8] (A85389) at a dilution of 1:4,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). The Anti-TDP43 Antibody [3H8] (A85389) strongly and specifically labels nuclei. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed tissues.