Anti-TEAD3 Antibody (A12121)

Name: Anti-TEAD3 Antibody
Brand: Antibodies.com
SKU: A12121
Price: 280.0 GBP
Availability: InStock

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This product is discontinued

Anti-TEAD3 Antibody (A12121) has been discontinued and is no longer available.

View all TEAD3 Antibodies.

Specifications

Name

Anti-TEAD3 Antibody

Description

Rabbit polyclonal antibody to TEAD3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 130-330 of human TEAD3 (NP_003205.2).

Sequence

SSAQIVSASVLQNKFSPPSPLPQAVFSTSSRFWSSPPLLGQQPGPSQDIKPFAQPAYPIQPPLPPTLSSYEPLAPLPSAAASVPVWQDRTIASSRLRLLEYSAFMEVQRDPDTYSKHLFVHIGQTNPAFSDPPLEAVDVRQIYDKFPEKKGGLKELYEKGPPNAFFLVKFWADLNSTIQEGPGAFYGVSSQYSSADSMTIS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

49 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DTEF-1, TEA domain family member 3, TEAD-3, TEAD5, TEF5, Transcriptional enhancer factor TEF-5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-TEAD3 Antibody (A12121)

Western blot analysis of extracts of various cell lines, using Anti-TEAD3 Antibody (A12121) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunohistochemistry - Anti-TEAD3 Antibody (A12121)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-TEAD3 Antibody (A12121) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-TEAD3 Antibody (A12121)

Immunofluorescence analysis of A549 cells using Anti-TEAD3 Antibody (A12121).

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Immunofluorescence - Anti-TEAD3 Antibody (A12121)

Confocal immunofluorescence analysis of U-2 OS cells using Anti-TEAD3 Antibody (A12121) at a dilution of 1:400. DAPI was used to stain the cell nuclei (blue).