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Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265)

Western Blot - Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265) - Antibodies.com Western Blot - Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265) - Antibodies.com Chromatin Immunoprecipitation - Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1157] antibody to TEF1/TEAD-1 for WB, IP and ChIP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-TEF1/TEAD-1 Antibody [ARC1157]
Description
Rabbit monoclonal [ARC1157] antibody to TEF1/TEAD-1.
Applications
WB, IP, ChIP
Dilutions
WB: 1:500-1:2,000, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TEAD1 (P28347).
Sequence
MEPSSWSGSESPAENMERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARR
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1157
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265) - Antibodies.comEnlarge Image

Western Blot - Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265)

Western blot analysis of extracts of various cell lines, using Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265) - Antibodies.comEnlarge Image

Western Blot - Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265)

Immunoprecipitation analysis of 600µg extracts of mouse skeletal muscle cells using 3µg of Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265). This Western blot was performed on the immunoprecipitate using Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265) at a dilution of 1:1000.
Chromatin Immunoprecipitation - Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265) - Antibodies.comEnlarge Image

Chromatin Immunoprecipitation - Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265)

Chromatin immunoprecipitation (ChIP) analysis of extracts of HepG2 cells, using Anti-TEF1/TEAD-1 Antibody [ARC1157] (A307265) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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