Anti-Thioredoxin / TRX Antibody [2B1] (A279180)

Anti-Thioredoxin / TRX Antibody [2B1]

Mouse monoclonal [2B1] antibody to Thioredoxin / TRX.

This antibody recognises human thioredoxin, also known as ATL-derived factor or Surface-associated sulphydryl protein. Thioredoxin is a 105 amino acid ~12kDa dithiol oxidoreductase with powerful protein disulphide oxidoreductase activity containing a single thioredoxin domain (UniProt: P10599).Thioredoxin is involved in a number of cellular processes, including activation of the transcription factor NF-KB. Thioredoxin is highly expressed in the glandular cells of the GI tract and uterine tissues, also activated B and T-cells. Mouse anti Human thioredoxin antibody, clone 2B1 has been used successfully in a biotinylated format as a detection reagent in a thioredoxin sandwich ELISA as part of a study looking at thioredoxin levels in placental trophoblast cells , a study which also used the antibody for immunoprecipitation of thioredoxin as well as detection of thioredoxin by western blotting. Mouse anti Human thioredoxin antibody, clone 2B1 is also useful for the demonstration of thioredoxin expression on formalin fixed, paraffin embedded material by immunohistochemistry. Clone 2B1 has also been used as a capture reagent in a sandwich ELISA for thioredoxin.

WB, IHC-P, Flow Cytometry, ELISA, IHC-Fr

WB: 10 µg/ml - 50 µg/ml

Human, Marmoset

Recombinant human thioredoxin.

Mouse

Monoclonal

2B1

IgG1

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human thioredoxin antibody, clone 2B1 recognizes human thioredoxin, also known as ATL-derived factor or Surface-associated sulphydryl protein. Thioredoxin is a 105 amino acid ~12kDa dithiol oxidoreductase with powerful protein disulphide oxidoreductase activity containing a single thioredoxin domain (UniProt: P10599).Thioredoxin is involved in a number of cellular processes, including activation of the transcription factor NF-KB (Kelleher et al. 2014). Thioredoxin is highly expressed in the glandular cells of the GI tract and uterine tissues, also activated B and T-cells (Rubartelli et al. 1992).Mouse anti Human thioredoxin antibody, clone 2B1 has been used successfully in a biotinylated format as a detection reagent in a thioredoxin sandwich ELISA as part of a study looking at thioredoxin levels in placental trophoblast cells (Di Trapani et al. 1998), a study which also used the antibody for immunoprecipitation of thioredoxin as well as detection of thioredoxin by western blotting.Mouse anti Human thioredoxin antibody, clone 2B1 is also useful for the demonstration of thioredoxin expression on formalin fixed, paraffin embedded material by immunohistochemistry (Lincoln et al. 2003). Clone 2B1 has also been used as a capture reagent in a sandwich ELISA for thioredoxin (Perking et al. 1995).

ADF, ATL derived factor, ATL-derived factor, DKFZp686B1993, MGC61975, SASP, Surface associated sulphydryl protein, Surface-associated sulphydryl protein, testicular tissue protein Li 199, Thioredoxin, thioredoxin delta 3, THIO_HUMAN, TRDX, Trx, TRX 1, TRX1, TXN, TXN delta 3, TXN protein, zgc:92903