Anti-Thrombospondin 1 Antibody (A11512)

Name: Anti-Thrombospondin 1 Antibody
Brand: Antibodies.com
SKU: A11512
Price: 270.0 GBP
Availability: InStock

Enlarge Image

$355

Rabbit polyclonal antibody to Thrombospondin 1 for WB and IHC.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Thrombospondin 1 Antibody

Description

Rabbit polyclonal antibody to Thrombospondin 1.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 19-352 of human THBS1 (NP_003237.2).

Sequence

NRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Glycoprotein G, THBS1, Thrombospondin-1, TSP, TSP1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

Enlarge Image

Western Blot - Anti-Thrombospondin 1 Antibody (A11512)

Western blot analysis of extracts of Mouse liver, using Anti-Thrombospondin 1 Antibody (A11512) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Western Blot - Anti-Thrombospondin 1 Antibody (A11512)

Western blot analysis of extracts of Rat lung, using Anti-Thrombospondin 1 Antibody (A11512) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Immunohistochemistry - Anti-Thrombospondin 1 Antibody (A11512)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Thrombospondin 1 Antibody (A11512) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Immunohistochemistry - Anti-Thrombospondin 1 Antibody (A11512)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Thrombospondin 1 Antibody (A11512) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.