Anti-Thyroid Hormone Receptor Antibody [H43] (A305055)

Immunohistochemistry analysis of human thyroid cancer. The Primary Antibody used was Anti-Thyroid Hormone Receptor Antibody [H43] (A305055) at 1:100 for Overnight at 4°C, then 30 minutes at 37°C. The secondary antibody used was Goat Anti-Mouse IgG (H+L): FITC for 45 minutes at 37°C. Counterstain: DAPI for 3 min at room temperature. Magnification: 4X.

Western blot analysis of human Hep G2 Hepatoblastoma Cell lysate showing detection of Thyroid Hormone Receptor protein using Anti-Thyroid Hormone Receptor Antibody [H43] (A305055) at 1:500 for 2 hours at room temperature with shaking. Load: 10 ug. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Other Band(s): Higher molceular weight bands could be due to PTMs.

Immunocytochemistry/Immunofluorescence analysis of human differentiated SH-SY5Y, using Anti-Thyroid Hormone Receptor Antibody [H43] (A305055), at 1:250. The secondary antibody used was AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue).(A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) Thyroid Hormone Receptor Antibody. (D) Hoechst (E) Composite.

Immunohistochemistry analysis of mouse thyroid. The Primary Antibody used was Anti-Thyroid Hormone Receptor Antibody [H43] (A305055) at 1:100 for Overnight at 4°C, then 30 minutes at 37°C. The secondary antibody used was Goat Anti-Mouse IgG (H+L): FITC for 45 minutes at 37°C. Counterstain: DAPI for 3 min at room temperature. Magnification: 7.5X.