Anti-TIM 4 Antibody [ROS-JH9] (A320288)

Anti-TIM 4 Antibody [ROS-JH9]

Mouse monoclonal [ROS-JH9] antibody to TIM 4.

This antibody recognises chicken TIM-4 (T-cell immunoglobulin mucin 4).In mice, TIM-4 is largely expressed on antigen-presenting cells and involved in phagocytosis of apoptotic cells with, additionally, a regulatory role in T-cell responses. In chickens, the gene for TIM-4 codes for a minimum of five splice variants that vary between stains. Myeloid antigen-presenting cells and ?d T cells express chTIM4; in contrast to mammalian T cells which do not express TIM-4. Research conducted by Hu et al. using Mouse anti Chicken TIM-4 antibody, clone ROS-JH9, revealed that it can detect all isoforms of chicken TIM-4. Clone ROS-JH9 was found to stain bone marrow macrophages from J line birds, a cell population that was also positive for clone KUL01 MCA5770. Further work characterized a distinct TIM-4 bearing set of phagocytes in the bursa and showed that approximately 50% of blood monocytes in chicken express detectable levels of TIM-4.

Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Chicken

Recombinant Chicken TIM-4 extracellular domain (amino acids 1-209) fused to chicken IgFc region.

Mouse

Monoclonal

ROS-JH9

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Chicken TIM-4 antibody, clone ROS-JH9, recognizes chicken TIM-4 (T-cell immunoglobulin mucin 4).In mice, TIM-4 is largely expressed on antigen-presenting cells and involved in phagocytosis of apoptotic cells with, additionally, a regulatory role in T-cell responses. In chickens, the gene for TIM-4 codes for a minimum of five splice variants that vary between stains. Myeloid antigen-presenting cells and ?d T cells express chTIM4; in contrast to mammalian T cells which do not express TIM-4 (Hu et al. 2016).Research conducted by Hu et al. using Mouse anti Chicken TIM-4 antibody, clone ROS-JH9, revealed that it can detect all isoforms of chicken TIM-4. Clone ROS-JH9 was found to stain bone marrow macrophages from J line birds, a cell population that was also positive for clone KUL01 MCA5770 (Hu et al. 2016). Further work characterized a distinct TIM-4 bearing set of phagocytes in the bursa and showed that approximately 50% of blood monocytes in chicken express detectable levels of TIM-4 (Hu et al. 2019).

T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIM-4, TIM4, TIMD-4, TIMD4