Anti-TIP49A Antibody [ARC1247] (A306321)

Name: Anti-TIP49A Antibody [ARC1247]
Brand: Antibodies.com
SKU: A306321
Price: 340.0 GBP
Availability: InStock

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This product is discontinued

Anti-TIP49A Antibody [ARC1247] (A306321) has been discontinued and is no longer available.

View all TIP49A Antibodies.

Specifications

Name

Anti-TIP49A Antibody [ARC1247]

Description

Rabbit monoclonal [ARC1247] antibody to TIP49A.

Applications

WB, IHC, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ChIP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-456 of human RUVBL1/TIP49A/PONTIN (Q9Y265).

Sequence

IPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1247

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

51 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP-54, INO80 complex subunit H, INO80H, NMP 238, NMP238, Nuclear matrix protein 238, Pontin 52, RuvB-like 1, TIP49

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-TIP49A Antibody [ARC1247] (A306321)

Western blot analysis of extracts of various cell lines, using Anti-TIP49A Antibody (A306321) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunohistochemistry - Anti-TIP49A Antibody [ARC1247] (A306321)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-TIP49A Antibody (A306321) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Chromatin Immunoprecipitation - Anti-TIP49A Antibody [ARC1247] (A306321)

Chromatin immunoprecipitation (ChIP) analysis of extracts of HepG2 cells, using Anti-TIP49A Antibody (A306321) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.