Anti-TLR2 Antibody [1H11] (A320265)

Anti-TLR2 Antibody [1H11]

Mouse monoclonal [1H11] antibody to TLR2.

This antibody recognises CD282, also known as toll-like receptor 2 (TLR2).Toll like receptors are a family of pattern-recognition receptors involved in the activation and regulation of inflammatory and immune responses activated by invasion of host tissues by microbial pathogens. TLR2 plays a role in a range of infections including gram-positive bacteria, mycobacteria, and yeast. It is activated by PAMPs, signals to NF-kappa-B which in turn induces cytokine secretion and the inflammatory response.Clone 1H11 was used to characterize the expression of porcine TLR2; cells of the innate immune system - monocytes, macrophages, and granulocytes, but not on peripheral blood lymphocytes were found to express porcine TLR2. Swine neutrophils express low levels of TLR2. It is also expressed on epithelial cells lining the tracheobronchial and intestinal tracts, bile ducts in the liver and renal tubules, and on the basal layer of the epidermis (Alvarez et al 2008).

Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Porcine

NIH/3T3 mouse cells transfected with plasmid pTLR2-GFP, encoding the porcine TLR2 tagged with the green fluorescent protein (GFP) on the carboxy-terminal end.

Mouse

Monoclonal

1H11

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig CD282, clone 1H11 recognizes CD282, also known as toll-like receptor 2 (TLR2). Toll like receptors are a family of pattern-recognition receptors involved in the activation and regulation of inflammatory and immune responses activated by invasion of host tissues by microbial pathogens. TLR2 plays a role in a range of infections including gram-positive bacteria, mycobacteria, and yeast. It is activated by PAMPs, signals to NF-kappa-B which in turn induces cytokine secretion and the inflammatory response. Clone 1H11 was used to characterize the expression of porcine TLR2; cells of the innate immune system - monocytes, macrophages, and granulocytes, but not on peripheral blood lymphocytes were found to express porcine TLR2. Swine neutrophils express low levels of TLR2. It is also expressed on epithelial cells lining the tracheobronchial and intestinal tracts, bile ducts in the liver and renal tubules, and on the basal layer of the epidermis (Alvarez et al 2008).

CD282, TIL4, Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4