Anti-TMEM49 / VMP1 Antibody (A304853)

Western blot analysis of human A549 showing detection of 46.2kDa VMP1 protein using Anti-TMEM49 / VMP1 Antibody (A304853) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: A549 cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 minutes at room temperature. Color Development: TMB. Predicted/Observed Size: 46.2kDa.

Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-TMEM49 / VMP1 Antibody (A304853), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane, Vacuole Membrane. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VMP1 Antibody. (D) Composite.