Anti-TNFSF18 / GITRL Antibody [YGL386] (A282039)

Anti-TNFSF18 / GITRL Antibody [YGL386]

Rat monoclonal [YGL386] antibody to TNFSF18 / GITRL.

This antibody recognises mouse glucocorticoid-induced tumour necrosis factor receptor ligand (GITRL), a 20 kDa member of the tumour necrosis factor ligand superfamily, otherwise known as TNSF18. Mouse GITRL is predominantly expressed on immature and mature dendritic cells, macrophages and B cells. Expression of mouse GITRL can be transiently up regulated on these cells, following lipolysaccharide (LPS) stimulation, but protein expression is rapidly down modulated shortly after stimulation.GITRL can neutralise the suppressive effects of CD4+CD25+ regulatory T cells and can also provide a co-stimulatory signal for both naïve and primed T cells, suggesting a key role for GITRL in the regulation of T cell-mediated immunity.

Flow Cytometry, ELISA

Flow Cytometry: 1:25 - 1:50, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Mouse

Recombinant mouse GITRL.

Rat

Monoclonal

YGL386

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse GITRL antibody, clone YGL386 recognizes mouse glucocorticoid-induced tumour necrosis factor receptor ligand (GITRL), a 20 kDa member of the tumour necrosis factor ligand superfamily, otherwise known as TNSF18. Mouse GITRL is predominantly expressed on immature and mature dendritic cells, macrophages and B cells. Expression of mouse GITRL can be transiently up regulated on these cells, following lipolysaccharide (LPS) stimulation, but protein expression is rapidly down modulated shortly after stimulation.GITRL can neutralise the suppressive effects of CD4+CD25+ regulatory T cells and can also provide a co-stimulatory signal for both naïve and primed T cells, suggesting a key role for GITRL in the regulation of T cell-mediated immunity.