Mouse monoclonal [MEM-75] antibody to Transferrin Receptor (Biotin).
Specificity
This antibody reacts with an extracellular epitope of CD71 antigen, a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages, and erythroid precursors. It is lost on resting blood leukocytes. This antibody does not block binding of transferrin to the receptor.
Applications
Flow Cytometry, IP, ICC
Dilutions
Flow Cytometry: 2-5 µg/ml.
Reactivity
Human
Immunogen
NALM-6 human pre-B cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-75
Isotype
IgG1
Conjugate
Biotin
Purification
This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.
Concentration
1 mg/ml
Predicted MW
95 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Separation of cells stained with Anti-Transferrin Receptor Antibody [MEM-75] (Biotin) (A85651), (concentration in sample 0,6 µg/ml, Streptavidin APC, red-filled) from cells unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis (surface staining).
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