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Anti-TRIM25 / EFP Antibody (A308764)

Western Blot - Anti-TRIM25 / EFP Antibody (A308764) - Antibodies.com
This product is discontinued

Anti-TRIM25 / EFP Antibody (A308764) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-TRIM25 / EFP Antibody
Description
Rabbit polyclonal antibody to TRIM25 / EFP.
Applications
WB, IHC, IP
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100, IP: 1:50-1:100
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM25 (NP_005073.2).
Sequence
SPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
71 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-TRIM25 / EFP Antibody (A308764) - Antibodies.comEnlarge Image

Western Blot - Anti-TRIM25 / EFP Antibody (A308764)

Western blot analysis of extracts from normal (control) and TRIM25 knockout (KO) A-549 cells, using Anti-TRIM25 / EFP Antibody (A308764) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10S.