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Anti-TRIM25/EFP Antibody (A90890)

Western Blot - Anti-TRIM25/EFP Antibody (A90890) - Antibodies.com Immunofluorescence - Anti-TRIM25/EFP Antibody (A90890) - Antibodies.com Western Blot - Anti-TRIM25/EFP Antibody (A90890) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to TRIM25/EFP for WB, IHC, ICC/IF and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-TRIM25/EFP Antibody
Description
Rabbit polyclonal antibody to TRIM25/EFP.
Applications
WB, IHC, ICC/IF, IP
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM25 (NP_005073.2).
Sequence
SPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
72 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-TRIM25/EFP Antibody (A90890) - Antibodies.comEnlarge Image

Western Blot - Anti-TRIM25/EFP Antibody (A90890)

Western blot analysis of various lysates, using Anti-TRIM25/EFP Antibody (A90890) at 1:1,500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunofluorescence - Anti-TRIM25/EFP Antibody (A90890) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-TRIM25/EFP Antibody (A90890)

Immunofluorescence analysis of L929 cells using Anti-TRIM25/EFP Antibody (A90890) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-TRIM25/EFP Antibody (A90890) - Antibodies.comEnlarge Image

Western Blot - Anti-TRIM25/EFP Antibody (A90890)

Immunoprecipitation analysis of 200µg extracts of HepG2 cells using 3µg of Anti-TRIM25/EFP Antibody (A90890). This Western blot was performed on the immunoprecipitate using Anti-TRIM25/EFP Antibody (A90890) at a dilution of 1:1000.
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