Synthetic peptide corresponding to the C terminal region of human TRIM54.
Sequence
C-GSAGPEEERPDGP
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
45 kDa and 38 kDa
Predicted MW
43.1kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
MURF, MuRF-3, Muscle-specific RING finger protein, Muscle-specific RING finger protein 3, RING finger protein 30, RNF30, Tripartite motif-containing protein 54
TRIM54 expression in Human Skeletal Muscle lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-TRIM54 Antibody (A83909) at 1µg/ml and detected by chemiluminescence.
TRIM54 expression in Human Skeletal Muscle analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-TRIM54 Antibody (A83909) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
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Proteins predicted to interact with MURF3
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
