Rabbit polyclonal antibody to TrkB (Phospho-Tyr705)
Specificity
The antibody detects endogenous level of TrkB only when phosphorylated at tyrosine 705.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 705 (T-D-Y P-Y-R) derived from Human TrkB.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
140kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, NTRK2, Trk-B, TrkB tyrosine kinase, Tropomyosin-related kinase B
Western blot analysis of extracts from mouse brain tissue using TrkB (Phospho-Tyr705) Antibody #11328 and the same antibody preincubated with blocking peptide.
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