Rabbit polyclonal antibody to TrkB (Phospho-Tyr705)
Specificity
The antibody detects endogenous level of TrkB only when phosphorylated at tyrosine 705.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 705 (T-D-Y P-Y-R) derived from Human TrkB.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
140kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, NTRK2, Trk-B, TrkB tyrosine kinase, Tropomyosin-related kinase B
Western blot analysis of extracts from mouse brain tissue using TrkB (Phospho-Tyr705) Antibody #11328 and the same antibody preincubated with blocking peptide.
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