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Anti-Tryptophan rich protein Antibody (A15476)

Western Blot - Anti-Tryptophan rich protein Antibody (A15476) - Antibodies.com Immunohistochemistry - Anti-Tryptophan rich protein Antibody (A15476) - Antibodies.com
This product is discontinued

Anti-Tryptophan rich protein Antibody (A15476) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-Tryptophan rich protein Antibody
Description
Rabbit polyclonal antibody to Tryptophan rich protein.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human WRB (NP_004618.2).
Sequence
SFSSFMSRVLQKDAEQESQMRAEIQDMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKI
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
25 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-Tryptophan rich protein Antibody (A15476) - Antibodies.comEnlarge Image

Western Blot - Anti-Tryptophan rich protein Antibody (A15476)

Western blot analysis of extracts of various cell lines, using Anti-Tryptophan rich protein Antibody (A15476) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Tryptophan rich protein Antibody (A15476) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Tryptophan rich protein Antibody (A15476)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Tryptophan rich protein Antibody (A15476) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.