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Anti-U2AF35/U2AF1 Antibody (A89592)

Western Blot - Anti-U2AF35/U2AF1 Antibody (A89592) - Antibodies.com Immunohistochemistry - Anti-U2AF35/U2AF1 Antibody (A89592) - Antibodies.com Western Blot - Anti-U2AF35/U2AF1 Antibody (A89592) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to U2AF35/U2AF1 for WB, IHC and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-U2AF35/U2AF1 Antibody
Description
Rabbit polyclonal antibody to U2AF35/U2AF1.
Applications
WB, IHC, IP
Dilutions
WB: 1:500-1:2,000, IHC: 1:100-1:200, IP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U2AF1 (NP_006749.1).
Sequence
MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQSADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMN
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
38 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-U2AF35/U2AF1 Antibody (A89592) - Antibodies.comEnlarge Image

Western Blot - Anti-U2AF35/U2AF1 Antibody (A89592)

Western blot analysis of extracts of various cell lines, using Anti-U2AF35/U2AF1 Antibody (A89592) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry - Anti-U2AF35/U2AF1 Antibody (A89592) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-U2AF35/U2AF1 Antibody (A89592)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-U2AF35/U2AF1 Antibody (A89592) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot - Anti-U2AF35/U2AF1 Antibody (A89592) - Antibodies.comEnlarge Image

Western Blot - Anti-U2AF35/U2AF1 Antibody (A89592)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-U2AF35/U2AF1 Antibody (A89592). This Western blot was performed on the immunoprecipitate using Anti-U2AF35/U2AF1 Antibody (A89592) at a dilution of 1:1000.
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