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Anti-UBE2J1 Antibody (A89338)

Western Blot - Anti-UBE2J1 Antibody (A89338) - Antibodies.com Immunofluorescence - Anti-UBE2J1 Antibody (A89338) - Antibodies.com Immunofluorescence - Anti-UBE2J1 Antibody (A89338) - Antibodies.com Immunofluorescence - Anti-UBE2J1 Antibody (A89338) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to UBE2J1 for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-UBE2J1 Antibody
Description
Rabbit polyclonal antibody to UBE2J1.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2J1 (NP_057105.2).
Sequence
METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPE
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
35 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CGI-76, HSPC153, HSPC205, HSU93243, HSUBC6e, MGC12555, NCUBE-1, NCUBE1, Non-canonical ubiquitin-conjugating enzyme 1, UB2J1_HUMAN, UBC6, UBC6e, Ubc6p, Ubiquitin conjugating enzyme E2, J1 (UBC6 homolog, yeast), Ubiquitin conjugating enzyme E2, J1, U, Ubiquitin-conjugating enzyme E2 J1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-UBE2J1 Antibody (A89338) - Antibodies.comEnlarge Image

Western Blot - Anti-UBE2J1 Antibody (A89338)

Western blot analysis of extracts of various cell lines, using Anti-UBE2J1 Antibody (A89338) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-UBE2J1 Antibody (A89338) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-UBE2J1 Antibody (A89338)

Immunofluorescence analysis of C6 cells using Anti-UBE2J1 Antibody (A89338) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-UBE2J1 Antibody (A89338) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-UBE2J1 Antibody (A89338)

Immunofluorescence analysis of HeLa cells using Anti-UBE2J1 Antibody (A89338) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-UBE2J1 Antibody (A89338) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-UBE2J1 Antibody (A89338)

Immunofluorescence analysis of NIH/3T3 cells using Anti-UBE2J1 Antibody (A89338) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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