Ube2L3 expression in HeLa cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Ube2L3 Antibody (A83899) at 0.5µg/ml and detected by chemiluminescence.
Ube2L3 expression in Human (A), Mouse (B), Rat (C), and Pig (D) Heart cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Ube2L3 Antibody (A83899) at 0.01µg/ml and detected by chemiluminescence.
Ube2L3 expression in Human (A), Mouse (B), and Rat (C) Skeletal Muscle cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Ube2L3 Antibody (A83899) at 0.03µg/ml and detected by chemiluminescence.
Ube2L3 expression in HEK293 (A), HepG2 (B), and Jurkat (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Ube2L3 Antibody (A83899) at 0.01µg/ml and detected by chemiluminescence.
Ube2L3 expression in MCF7 (A) and HeLa (B) cells analyzed by immunofluorescence. Staining was performed with Anti-Ube2L3 Antibody (A83899) at 5µg/ml and Rabbit Anti-goat IgG-Alexa Fluor 488 secondary antibody at 1:1000 with DAPI nuclear counterstaining.