Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
E3 UFM1-protein ligase 1, E3 UFM1-protein transferase 1, KIAA0776, MAXER, Multiple alpha-helix protein located at ER, NLBP, Novel LZAP-binding protein, RCAD, Regulator of C53/LZAP and DDRGK1
Western blot analysis of extracts of HepG2 cells, using Anti-UFL1 Antibody (A305259) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Anti-UFL1 Antibody (A305259) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-UFL1 Antibody (A305259) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-UFL1 Antibody (A305259) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
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Proteins predicted to interact with UFL1
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
