Anti-ULK1 Antibody (A10448)

Name: Anti-ULK1 Antibody
Brand: Antibodies.com
SKU: A10448
Price: 280.0 GBP
Availability: InStock

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This product is discontinued

Anti-ULK1 Antibody (A10448) has been discontinued and is no longer available.

View all ULK1 Antibodies.

Specifications

Name

Anti-ULK1 Antibody

Description

Rabbit polyclonal antibody to ULK1.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ULK1 (NP_003556.2).

Sequence

GKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

150 kDa / 140 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ATG1, Autophagy-related protein 1 homolog, hATG1, KIAA0722, Serine/threonine-protein kinase ULK1, Unc-51-like kinase 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-ULK1 Antibody (A10448)

Western blot analysis of extracts of Rat brain, using Anti-ULK1 Antibody (A10448) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit. Exposure time: 180s.

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Western Blot - Anti-ULK1 Antibody (A10448)

Western blot analysis of extracts of various lysates, using Anti-ULK1 Antibody (A10448) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunofluorescence - Anti-ULK1 Antibody (A10448)

Immunofluorescence analysis of HeLa cells using Anti-ULK1 Antibody (A10448) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-ULK1 Antibody (A10448)

Immunofluorescence analysis of NIH/3T3 cells using Anti-ULK1 Antibody (A10448) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).