Anti-USP25 Antibody (A16016)

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This product is discontinued

Anti-USP25 Antibody (A16016) has been discontinued and is no longer available.

View all USP25 Antibodies.

Specifications

Name

Anti-USP25 Antibody

Description

Rabbit polyclonal antibody to USP25.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2000, IHC: 1:50-1:200, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human USP25 (NP_037528.3).

Sequence

MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

130kDa

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Deubiquitinating enzyme 25, Ubiquitin carboxyl-terminal hydrolase 25, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25, USP on chromosome 21, USP21

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-USP25 Antibody (A16016)

Western blot analysis of extracts of various cell lines, using Anti-USP25 Antibody (A7975) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

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Western Blot - Anti-USP25 Antibody (A16016)

Western blot analysis of extracts of various cells, using Anti-USP25 Antibody (A7975) at 1:1,000 dilution ratio through one-step method. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:4000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.