Anti-VAPB Antibody (A14703)

Name: Anti-VAPB Antibody
Brand: Antibodies.com
SKU: A14703

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This product is discontinued

Anti-VAPB Antibody (A14703) has been discontinued and is no longer available.

View all VAPB Antibodies.

Specifications

Name

Anti-VAPB Antibody

Description

Rabbit polyclonal antibody to VAPB.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 127-222 of human VAPB (NP_004729.1).

Sequence

AENDKPHDVEINKIISTTASKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDGLRMRKTVQSNSPISALAPTGKEEGLST

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

27 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

VAMP-associated protein B/C, VAMP-B/VAMP-C, VAP-B/VAP-C, Vesicle-associated membrane protein-associated protein B/C

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-VAPB Antibody (A14703)

Western blot analysis of extracts of various cell lines, using Anti-VAPB Antibody (A14703) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-VAPB Antibody (A14703)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-VAPB Antibody (A14703) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-VAPB Antibody (A14703)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-VAPB Antibody (A14703) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-VAPB Antibody (A14703)

Immunofluorescence analysis of U2OS cells using Anti-VAPB Antibody (A14703) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).