Anti-VDAC1 / Porin Antibody [S152B-23] (A304822)

Western blot analysis of mouse brain and human room temperature-4 lysates showing detection of ~30 kDa VDAC1 protein using Anti-VDAC1 / Porin Antibody [S152B-23] (A304822) at 1:200 for 60 minutes at room temperature. Lane 1: Molecular Weight Ladder. Lane A: mouse brain Lysate. Lane B: human room temperature-4 Lysate. Block: 5% Skim milk in TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: ~30 kDa.

Western blot analysis of rat brain membrane showing detection of ~30 kDa VDAC1 protein using Anti-VDAC1 / Porin Antibody [S152B-23] (A304822) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: rat brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~30 kDa.

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-VDAC1 / Porin Antibody [S152B-23] (A304822), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VDAC1 Antibody (D) Composite.