Anti-VHL Antibody [AMC0442] (A310017)

Name: Anti-VHL Antibody [AMC0442]
Brand: Antibodies.com
SKU: A310017
Price: 295.0 GBP
Availability: InStock

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$385

Mouse monoclonal [AMC0442] antibody to VHL for WB, IHC-P and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-VHL Antibody [AMC0442]

Description

Mouse monoclonal [AMC0442] antibody to VHL.

Applications

WB, IHC-P, ELISA

Positive Control

HeLa, Raji, 293T, Mouse embryo, Rat testis.

Dilutions

WB: 1:500-1:1,000, IHC-P: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 1-154 of human VHL.

Sequence

MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLP

Host

Mouse

Clonality

Monoclonal

Clone ID

AMC0442

Isotype

IgG2b

Light Chains

kappa

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

18 kDa

Predicted MW

24 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Protein G7, pVHL, von Hippel-Lindau disease tumor suppressor

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-VHL Antibody [AMC0442] (A310017)

VHL expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-VHL Antibody [AMC0442] (A310017) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunohistochemistry - Anti-VHL Antibody [AMC0442] (A310017)

VHL expression in human brain tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-VHL Antibody [AMC0442] (A310017) at a dilution of 1:200.

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Immunohistochemistry - Anti-VHL Antibody [AMC0442] (A310017)

VHL expression in human liver cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-VHL Antibody [AMC0442] (A310017) at a dilution of 1:200.

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Immunohistochemistry - Anti-VHL Antibody [AMC0442] (A310017)

VHL expression in human pancreas tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-VHL Antibody [AMC0442] (A310017) at a dilution of 1:200.