Anti-Vimentin Antibody [VM452] (CF® 488) (A251156)

Anti-Vimentin Antibody [VM452] (CF® 488)

Mouse monoclonal [VM452] antibody to Vimentin (CF® 488).

This antibody reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Flow Cytometry, IF

Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100

Human, Bovine, Canine, Feline, Porcine, Goat, Chicken

This antibody does not cross react with Mouse or Rat.

Recombinant human Vimentin protein.

Mouse

VM452

IgG1

kappa

Protein A/G chromatography.

100 µg/ml

57-60 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Store at 4-8°C. Antibody is stable for 24 months.

VIM

This product is for research use only. It is not intended for diagnostic or therapeutic use.