Anti-VPS15 Antibody [ARC2090] (A305330)

Name: Anti-VPS15 Antibody [ARC2090]
Brand: Antibodies.com
SKU: A305330
Price: 340.0 GBP
Availability: InStock

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This product is discontinued

Anti-VPS15 Antibody [ARC2090] (A305330) has been discontinued and is no longer available.

View all VPS15 Antibodies.

Specifications

Name

Anti-VPS15 Antibody [ARC2090]

Description

Rabbit monoclonal [ARC2090] antibody to VPS15.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1259-1358 of human PIK3R4/VPS15 (Q99570).

Sequence

AGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2090

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

153 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Phosphoinositide 3-kinase adaptor protein, Phosphoinositide 3-kinase regulatory subunit 4, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, PIK3R4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-VPS15 Antibody [ARC2090] (A305330)

Western blot analysis of extracts of various cell lines, using Anti-VPS15 Antibody (A305330) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Western Blot - Anti-VPS15 Antibody [ARC2090] (A305330)

Western blot analysis of extracts of Rat brain, using Anti-VPS15 Antibody (A305330) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunofluorescence - Anti-VPS15 Antibody [ARC2090] (A305330)

Immunofluorescence analysis of HeLa cells using Anti-VPS15 Antibody (A305330) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-VPS15 Antibody [ARC2090] (A305330)

Immunofluorescence analysis of NIH-3T3 cells using Anti-VPS15 Antibody (A305330) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).