Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Dermal papilla-derived protein 9, DERP9, EAP20, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, hVps25, Vacuolar protein-sorting-associated protein 25
Western blot analysis of extracts of various cell lines, using Anti-VPS25 Antibody (A88704) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of C6 cells using Anti-VPS25 Antibody (A88704) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH-3T3 cells using Anti-VPS25 Antibody (A88704) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-VPS25 Antibody (A88704)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-VPS25 Antibody (A88704)
