Anti-WIPI2 Antibody [2A2] (A279175)

Anti-WIPI2 Antibody [2A2]

Mouse monoclonal [2A2] antibody to WIPI2.

This antibody recognises both WIPI2A and WIPI2B by western blotting Mouse anti Human WIPI2 antibody, clone 2A2 has been used for the immunofluorescent detection of WIPI2 in the human retinal epithelial cell line RPE1.

WB, IHC-P, IF, IP

Human, Mouse

Synthetic peptide sequence CSALRLDEDSEHPPMILRTD corresponding to the C-terminus of WIPI2b.

CSALRLDEDSEHPPMILRTD

Mouse

Monoclonal

2A2

IgG1

1 mg/ml

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human WIPI2 antibody, clone 2A2 recognies WD repeat domain phosphoinositide-interacting protein 2 (WIPI-2), also known as WIPI49-like protein 2. WIPI2 is a 454 amino acid ~54 kDa autophagosomal marker containing three WD repeats. WIPI2 is a mammalian orthologue of the yeast protein Atg18 and is similarly recruited to early autophagosomal structures and is required for their maturation into mature autophagosomes (Polson et al. 2010).Human WIPI2 exists in multiple isoforms including WIPI2A, the canonical 454 amino acid isoform and WIPI2B with deletions towards both the N and C terminal regions. Mouse anti Human WIPI2 antibody, clone 2A2 was generated using a C-terminal sequence and recognizes both WIPI2A and WIPI2B by western blotting (Pantoom et al. 2020)Mouse anti Human WIPI2 antibody, clone 2A2 has been used for the immunofluorescent detection of WIPI2 in the human retinal epithelial cell line RPE1 (MacVicar and Lane 2014).

WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2