Anti-Wnt3 Antibody (A320092)

Anti-Wnt3 Antibody

Goat polyclonal antibody to Wnt3.

This antibody recognises both human and mouse proto-oncogene Wnt-3, a member of the Wnt family, and ligand of the frizzled family of seven transmembrane receptors.Wnt-3, otherwise known as Int-4, is involved in cell fate decisions and patterning during embryogenesis, cell signalling during neural tube development, and implicated in the tumorogenesis of several cancers including breast, lung, hepatocellular(HCC), and gastric carcinoma.Over-expression of Wnt-3 playes a pivotal role in the resistance of HER2-overexpressing breast tumors to the breast cancer treatment drug Trastuzumab, also known as Herceptin. The effects of Wnt-3 over-expression included the transactivated expression of EGFR (epidermal growth factor receptor) an increased expression of B-catenin within the nucleus, an increase in N-cadherin and the neural crest transcription factor SLUG, as well as promoting an EMT (epithelial to mesenchymal transition) response.Goat anti Wnt-3 polyclonal antibody exhibits strong fibrous staining in myoepithelial cells of human breast tissue in paraffin sections.Goat anti Wnt-3 polyclonal antibody stains mouse hippocampal primary astrocytes in ICC/IF.

WB, IHC-P, ELISA, IF

ELISA: 1:16,000, IHC-P: 3.0 - 5.0 µg/ml, WB: 1.0 - 3.0 µg/ml

Human, Mouse, Dog, Rat

Synthetic peptide sequence C-GRGHNTRTEKRKEK from the internal region of Wnt-3 (NP_110380.1).

C-GRGHNTRTEKRKEK

Goat

Polyclonal

IgG

500 µg/ml

Approximately 40 kDa in HEK293 cell lysates

Liquid

Supplied in TRIS Buffered Saline, 0.5% BSA and 0.02% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Goat anti Wnt-3 antibody recognises both human and mouse proto-oncogene Wnt-3, a member of the Wnt family, and ligand of the frizzled family of seven transmembrane receptors.Wnt-3, otherwise known as Int-4, is involved in cell fate decisions and patterning during embryogenesis, cell signalling during neural tube development, and implicated in the tumorogenesis of several cancers including breast, lung, hepatocellular(HCC), and gastric carcinoma.Over-expression of Wnt-3 playes a pivotal role in the resistance of HER2-overexpressing breast tumors to the breast cancer treatment drug Trastuzumab, also known as Herceptin (Wu et al. 2012). The effects of Wnt-3 over-expression included the transactivated expression of EGFR (epidermal growth factor receptor) an increased expression of B-catenin within the nucleus, an increase in N-cadherin and the neural crest transcription factor SLUG, as well as promoting an EMT (epithelial to mesenchymal transition) response.Goat anti Wnt-3 polyclonal antibody exhibits strong fibrous staining in myoepithelial cells of human breast tissue in paraffin sections.Goat anti Wnt-3 polyclonal antibody stains mouse hippocampal primary astrocytes in ICC/IF (Okamoto et al. 2011).

INT4, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3