Rabbit polyclonal antibody to WWOX (Phospho-Tyr33)
Specificity
The antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 33 (W-V-Y(p)-Y-A) derived from Human WWOX.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
FOR, Fragile site FRA16D oxidoreductase, SDR41C1, Short chain dehydrogenase/reductase family 41C member 1, WOX1, WW domain-containing oxidoreductase
Western blot analysis of extracts from HepG2 cells treated with PMA using WWOX (Phospho-Tyr33) Antibody #11779.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody #11779 (left)or the same antibody preincubated with blocking peptide (right).
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