Rabbit polyclonal antibody to WWOX (Phospho-Tyr33)
Specificity
The antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 33 (W-V-Y(p)-Y-A) derived from Human WWOX.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
FOR, Fragile site FRA16D oxidoreductase, SDR41C1, Short chain dehydrogenase/reductase family 41C member 1, WOX1, WW domain-containing oxidoreductase
Western blot analysis of extracts from HepG2 cells treated with PMA using WWOX (Phospho-Tyr33) Antibody #11779.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody #11779 (left)or the same antibody preincubated with blocking peptide (right).
Alternative products to Anti-WWOX (Phospho-Tyr33) Antibody (A50503)
