Anti-XPA Antibody (A13558)

Enlarge Image

This product is discontinued

Anti-XPA Antibody (A13558) has been discontinued and is no longer available.

View all XPA Antibodies.

Specifications

Name

Anti-XPA Antibody

Description

Rabbit polyclonal antibody to XPA.

Applications

WB, IF

Dilutions

WB: 1:500-1:2000, IF: 1:50-1:100

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human XPA (NP_000371.1).

Sequence

MAAADGALPEAAALEQPAELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEYGPEENLEDDMYRKTCTMCGHELTYEKM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

37kDa

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA repair protein complementing XP-A cells, Xeroderma pigmentosum group A-complementing protein, XPAC

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

Enlarge Image

Western Blot - Anti-XPA Antibody (A13558)

Western blot analysis of extracts of various cell lines, using Anti-XPA Antibody (A1626) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST.

Enlarge Image

Western Blot - Anti-XPA Antibody (A13558)

Western blot analysis of extracts from normal (control) and XPA knockout (KO) HeLa cells, using Anti-XPA Antibody (A1626) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Enlarge Image

Immunofluorescence - Anti-XPA Antibody (A13558)

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Anti-XPA Antibody (A1626). Green: GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.