Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
DNA excision repair protein ERCC-4, DNA repair endonuclease XPF, DNA repair protein complementing XP-F cells, ERCC11, ERCC4, Xeroderma pigmentosum group F-complementing protein
Western blot analysis of extracts of various cell lines, using Anti-XPF Antibody (A12212) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation analysis of 150µg extracts of 293T cells using 3µg of Anti-XPF Antibody (A12212). This Western blot was performed on the immunoprecipitate using Anti-XPF Antibody (A12212) at a dilution of 1:1000.
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