Anti-Y14 Antibody [PCRP-RBM8A-1B4] (A250813)

Anti-Y14 Antibody [PCRP-RBM8A-1B4]

Mouse monoclonal [PCRP-RBM8A-1B4] antibody to Y14.

Y14 Antibody is a high quality monoclonal Y14 antibody (also designated Y14 antibody) suitable for the detection of the Y14 protein of mouse, rat, human and Xenopus laevis origin. Y14 Antibody (4C4) is available as both the non-conjugated anti-Y14 antibody form, as well as multiple conjugated forms of anti-Y14 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor conjugates. The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include Y14, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and Y14, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, Y14, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human Y14 encodes three transcripts. Y14 is a ubiquitously expressed protein. Although Y14 shuttles to the cytoplasm, it is predominantly detected in the nucleus and is co-localized with oskar mRNA at the posterior pole of the cell.

Flow Cytometry, IF, WB

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml

Human

Recombinant full-length human RBM8A protein.

Mouse

Monoclonal

PCRP-RBM8A-1B4

IgG1

Protein A/G chromatography.

200 µg/ml

24 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Y14 Antibody [PCRP-RBM8A-1B4] - BSA and Azide free (A253993).

Binder of OVCA1-1, BOV-1, RBM8, RBM8A, Ribonucleoprotein RBM8A, RNA-binding motif protein 8A, RNA-binding protein 8A, RNA-binding protein Y14