YTHDC2 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-YTHDC2 Antibody (A329971) at a dilution of 1:1000 and detected with chemiluminescence.
YTHDC2 expression in lysates from wild type (WT) and YTHDC2 knockdown (KD) HeLa cells analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-YTHDC2 Antibody (A329971) at a dilution of 1:1000 and detected with chemiluminescence.
YTHDC2 expression in paraffin-embedded Mouse testis tissue analyzed by immunofluorescence. Staining was performed with Anti-YTHDC2 Antibody (A329971) at a dilution of 1:150 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
YTHDC2 expression in paraffin-embedded Rat testis tissue analyzed by immunofluorescence. Staining was performed with Anti-YTHDC2 Antibody (A329971) at a dilution of 1:150 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
YTHDC2 expression in 200 µg extracts from 293T cells analyzed by western blot following immunoprecipitation using Anti-YTHDC2 Antibody (A329971) at a dilution of 1:1000 and using 0.5 µg, respectively.