Anti-YTHDF1 Antibody (A90730)

Name: Anti-YTHDF1 Antibody
Brand: Antibodies.com
SKU: A90730
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to YTHDF1 for WB, ICC/IF, IP and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-YTHDF1 Antibody

Description

Rabbit polyclonal antibody to YTHDF1.

Applications

WB, ICC/IF, IP, ELISA

Positive Control

Jurkat, C6.

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200, IP: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 1-200 of human YTHDF1.

Sequence

MSATSVDTQRTKGQDNKVQNGSLHQKDTVHDNDFEPYLTGQSNQSNSYPSMSDPYLSSYYPPSIGFPYSLNEAPWSTAGDPPIPYLTTYGQLSNGDHHFMHDAVFGQPGGLGNNIYQHRFNFFPENPAFSAWGTSGSQGQQTQSSAYGSSYTYPPSSLGGTVVDGQPGFHSDTLSKAPGMNSLEQGMVGLKIGDVSSSAV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

70 kDa

Predicted MW

61 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

C20orf21, DACA-1, Dermatomyositis associated with cancer putative autoantigen 1, DF1, YTH domain-containing family protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-YTHDF1 Antibody (A90730)

YTHDF1 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-YTHDF1 Antibody (A90730) at a dilution of 1:500 and detected with chemiluminescence.

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Immunofluorescence - Anti-YTHDF1 Antibody (A90730)

YTHDF1 expression in L929 cells analyzed by immunofluorescence. Staining was performed with Anti-YTHDF1 Antibody (A90730) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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Immunofluorescence - Anti-YTHDF1 Antibody (A90730)

YTHDF1 expression in U2OS cells analyzed by immunofluorescence. Staining was performed with Anti-YTHDF1 Antibody (A90730) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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