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Anti-Zap70 + Syk (phospho Tyr319 & Phospho Tyr352) Antibody (A329878)

Immunofluorescence - Anti-Zap70 + Syk (Phospho Y319 & Phospho Y352) Antibody (A329878) - Antibodies.com
$515
Product Datasheet

Rabbit polyclonal antibody to Zap70 + Syk (phospho Tyr319 & phospho Tyr352) for WB, ICC/IF and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Zap70 + Syk (phospho Tyr319 & Phospho Tyr352) Antibody
Description
Rabbit polyclonal antibody to Zap70 + Syk (phospho Tyr319 & phospho Tyr352).
Applications
WB, ICC/IF, ELISA
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)
Reactivity
Mouse
Immunogen
A synthetic peptide phosphorylated at Y319 of human Zap70 + Syk.
Sequence
SPY(p)SD
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
70kDa
Predicted MW
70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Immunofluorescence - Anti-Zap70 + Syk (Phospho Y319 & Phospho Y352) Antibody (A329878) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Zap70 + Syk (phospho Tyr319 & Phospho Tyr352) Antibody (A329878)

Confocal imaging of EL4 cells (treated with H2O2 and pervanadate) and EL4 cells (untreated) using Anti-Zap70 + Syk (Phospho Y319 & Phospho Y352) Antibody (A329878) at a dilution of 1:100 following further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) at a dilution of 1:500 (red). Nuclei were stained with DAPI (blue). Objective: 100x.
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