Native Bovine GFAP Protein (A270562)

SDS-PAGE gel of Native Bovine GFAP Protein (A270562); with three lanes of increasing load size.

SDS-PAGE of Native Bovine GFAP Protein (A270562) isolated from bovine spinal cord using the the method of Leung and Liem as far as the 6M urea/hydroxyapatite step. The material was then fractionated using a phosphate gradient on DEAE-cellulose in 6M urea, and GFAP rich fractions were concentrated. The lanes contain: [Lane S] protein standard, [Next three lanes] indicated amounts of BSA standard, [Lanes 1, 2 and 3] purified bovine GFAP, each with half the amount of the previous. There are multiple isotypes of GFAP generated by alternate transcription from a single gene, and there are several possible post-translational modifications of the expressed isoforms. As a result, these preparations do not show a single protein band, but several, all of which can be bound with most monoclonal antibodies to GFAP.