Reconstitute with phosphate buffer pH >7.0 containing 0.15 M NaCl. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Purified human albumin is a ~66 kDa protein that binds and transports steroids, fatty acids, metal ions and thyroid hormones. Albumin accounts for approximately 50% of blood serum protein.
Synonyms
ALB
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Sandwich ELISA analysis of albumin binding using Anti-Albumin Antibody [BGN/1328/33 (UA33)] (A279826) as a capture reagent and Anti-Human Serum Albumin Antibody (A283220) as a detection reagent with Native Human Albumin Protein (A320432) as antigen. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. Plasma sample is displayed at 30000 (Green) and 60000 (Red) fold dilutions.
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