Reconstitute with urea/Tris buffer (20mM Tris, pH 7.5, 5 mM EDTA, 7 M urea and 15 mM 2-mercaptoethanol). It is recommended to reconstitute to not less than 100 µg/ml, which can then be further diluted.
Storage
Shipped at 4°C. Short term (1-2 weeks) store at room temperature. Long term store desiccated below -18°C. Avoid freeze/thaw cycles. Upon reconstitution, store at 4°C (for up to 1 week) and below -18°C for future use.
General Notes
A blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.
