>95% Single band in high resolution protein electrophoresis on Sebia agarose gel. Confirmation of IgM and kappa light chain via Sebia agarose immunofixation.
Purification
Delipidation, salt fractionation and ion exchange chromatography.
Product Form
Lyophilized
Concentration
5 mg/ml after reconstitution.
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Store undiluted at -20°C only, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Sandwich ELISA analysis of IgM binding using Anti-Human IgM Antibody [M15/8] (A280995) as a capture reagent and Goat Anti-Human IgM Antibody (A283206) as a detection regent with Native Human IgM Protein (A320021) as antigen for the generation of the standard curve. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on a Microplate Absorbance Reader. Serum samples at 1:10k (green) and 1:100k (blue), similarly plasma samples 1:10k (orange) and 1:100k (red) dilutions are shown.
Sandwich ELISA analysis of IgM binding using Anti-Human IgM Antibody [M15/8] (A280995) as a capture reagent and Goat Anti-Human IgM Antibody (A283206) as a detection regent with Native Human IgM Protein (A320021) as antigen for the generation of the standard curve. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on a Microplate Absorbance Reader. Serum samples at 1:10k (green) and 1:100k (blue), similarly plasma samples 1:10k (orange) and 1:100k (red) dilutions are shown.
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