One unit is defined as the amount of enzyme required to cleave 50µg of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25mM Tris-HCl, pH 7.6, 50mM NaCl, and 2mM CaCl2.
Purity
>95% as determined by SDS-PAGE.
Product Form
Liquid
Concentration
Lot Specific
Formulation
Supplied in 50 mM Tris HCl Buffer, pH 8, with 500 mM Sodium Chloride and 50% Glycerol.
Storage
Shipped at 4°C. Long term (1 year) store at -20°C. Avoid freeze/thaw cycles.