Recombinant E. coli Ribonuclease H Protein (Functional)
Applications
SDS-PAGE, Functional Studies
Applications
Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA.
Removal of poly (A) tails from mRNA after hybridization with oligo (dT).
Oligodeoxyribonucleotide-directed site-specific cleavage of RNA.
Expression System
Escherichia coli
Nature
Recombinant
Protein Species
Escherichia coli
Protein Length
Full length protein
Molecular Weight
17.6 kDa
Biologically Active
Yes
Biological Activity
~100,000 units/mg protein.
Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of the RNA in 3H-labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37°C.
Purity
> 95% (by SDS-PAGE). The absence of endonucleases and exonucleases was confirmed.
Product Form
Liquid
Concentration
50 units/µl
Formulation
Supplied in 20mM Tris-HCl, pH 7.5, with 100mM KCl, 1mM DTT, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
H1RNA, MGC108918, Ribonuclease H type II, Ribonuclease HI, RibonucleaseH1, RNase H, RNase H1, RNase HI, RNaseH1, RNH1, rnhA
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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