Recombinant Human 68kDa Neurofilament/NF-L Protein (6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
6xHis Tag + NEFL (Gln89-Leu400)
Tag
N-terminal 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 36.9 kDa after removal of the signal peptide. The apparent molecular mass of His-NEFL(89-400) is approximately 35-55 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 85% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Human 68kDa Neurofilament/NF-L Protein (6xHis Tag) (A318095)
ELISA plates were pre-coated with 1 µg/ml (100 µl/well) Recombinant Human 68kDa Neurofilament/NF-L Protein (6×His Tag) (A318095) which can bind Anti-68kDa Neurofilament (89-400)/NF-L (89-400) Antibody [DM199] - Azide free (A318510) in a linear range of 0.128-80 µg/ml.
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