Recombinant Human 68kDa Neurofilament/NF-L Protein (Fc Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
NEFL (Thr9-Thr88) + hFc (Glu99-Ala330)
Tag
C-terminal Human Fc Tag
Molecular Weight
The protein has a predicted molecular mass of 34.9 kDa after removal of the signal peptide. The apparent molecular mass of NEFL(9-88)-hFc is approximately 35-55 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Human 68kDa Neurofilament/NF-L Protein (Fc Tag) (A318090)
ELISA plates were pre-coated with 1 µg/ml (100 µl/well) Recombinant Human 68kDa Neurofilament/NF-L Protein (Fc Tag) (A318090) which can bind Anti-68kDa Neurofilament (9-88)/NF-L (9-88) Antibody [DM198] - Azide free (A318511) in a linear range of 0.128-80 µg/ml.
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