Inhibition of chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2: IC50 = <11.5 nM.
Purity
>97% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 20mM HEPES, pH 8.0, with 150mM NaCl (0.22µm filter sterilized).
Reconstitution
Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
SDS-PAGE - Recombinant Human AACT Protein (C-terminal His Tag) (A330062)
SDS-PAGE of Recombinant Human AACT Protein (C-terminal His Tag) (A330062) using Coomassie Blue to show a band at 55-70 kDa.
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Proteins predicted to interact with alpha 1 Antichymotrypsin
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
