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Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744)

Tris-Bis PAGE - Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744) - Antibodies.com SEC-HPLC - Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744) - Antibodies.com Standard Curve - Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744) - Antibodies.com SPR Assay - Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744) - Antibodies.com
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag)
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Tag
C-terminal His and Avi Tag
Conjugate

Unconjugated

Purity
>95% (by Tris-Bis PAGE and HPLC).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Tris-Bis PAGE - Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744) - Antibodies.comEnlarge Image

Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744)

Tris-Bis PAGE of Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744) under non-reducing (N) conditions confirms that purity is greater than 95%.
SEC-HPLC - Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744) - Antibodies.comEnlarge Image

Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744)

SEC-HPLC analysis of Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744) confirms that purity is greater than 95%.
Standard Curve - Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744) - Antibodies.comEnlarge Image

Standard Curve - Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744)

ELISA examination of immobilized Human MAGE-A4 (HLA-A*02:01) Tetramer, His Tag, (0.5µg/mL) binding Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744): EC50 =6.1ng/ml.
SPR Assay - Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744) - Antibodies.comEnlarge Image

Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744)

Recombinant Human AFP + HLA-A*02:01 Protein (C-terminal His and Avi Tag) (A331744) binding to Anti-MAGE-A4 (HLA-A*02:01) Antibody (Human Fc Tag) on a CM5 Chip via Protein A, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 8.49pM.
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