Recombinant Human BAFF Protein (A330142)

Name: Recombinant Human BAFF Protein
Brand: Antibodies.com
SKU: A330142
Price: 130.0 GBP
Availability: InStock

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$180

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human BAFF Protein

Expression System

Escherichia coli

Nature

Recombinant

Protein Species

Human

Sequence

AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

Conjugate

Unconjugated

Biological Activity

Immobilized recombinant human BAFF (5 µg/mL) bind recombinant human BCMA in a functional ELISA with a linear range of 3-20 ng/mL. Cell proliferation assay using anti-IgM stimulated mouse B cells: ED50 = 0.1-1 ng/mL; Specific activity =1×10^6-1×10^7units/mg. Loaded Human TNFRSF17/BCMA/CD269 Protein, C-Human Fc and His Tag, on ProA Biosensor, can bind Human TNFSF13B/BAFF/CD257 Protein in BLI assay (Gator) with an affinity constant of 3.05nM.

Purity

>90% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from 50mM Tris, pH 8.0, with 150mM NaCl and without preservatives or carriers (0.22µm filter sterilized).

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

B lymphocyte stimulator, B-cell-activating factor, BLYS, CD257, Dendritic cell-derived TNF-like molecule, TALL-1, TALL1, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, Tumor necrosis factor ligand superfamily member 13B, ZTNF4

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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SDS-PAGE - Recombinant Human BAFF Protein (A330142)

SDS-PAGE of Recombinant Human BAFF Protein (A330142) using Coomassie Blue to show a band at 20 kDa.

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Standard Curve - Recombinant Human BAFF Protein (A330142)

ELISA examination of immobilized recombinant human BAFF (5 µg/mL) binding Recombinant Human BAFF Protein (A330142): linear range = 3-20 ng/mL.

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Recombinant Human BAFF Protein (A330142)

Cell proliferation assay using Recombinant Human BAFF Protein (A330142) on Mouse B cell : ED50 = 0.1-1 ng/mL; Specific activity = 1×10^6-1×10^7units/mg.

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Recombinant Human BAFF Protein (A330142)

Recombinant Human BAFF Protein (A330142) binding to Human TNFRSF17/BCMA/CD269 Protein, C-Human Fc and His Tag, immobilized on ProA Biosensor, analyzed by bio-layer interferometry (BLI; Gator). Affinity constant = 3.05nM.

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